Barcoded primers used in multiplex amplicon pyrosequencing bias amplification.

نویسندگان

  • David Berry
  • Karim Ben Mahfoudh
  • Michael Wagner
  • Alexander Loy
چکیده

"Barcode-tagged" PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyrosequencing libraries.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 77 21  شماره 

صفحات  -

تاریخ انتشار 2011